Protein Variants | Comment | Organism |
---|---|---|
S175A | less than 0.01% of activity of the wild-type enzyme | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + acceptor | Escherichia coli | fourth step in synthesis of pyrimidine nucleotides | orotate + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
family 2 enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-dihydroorotate + a quinone = orotate + a quinol | existence of 3 families differing in their selectivity for oxidizing substrates: 1A are soluble, containing FMN and use fumarate, 1B are soluble, one subunit contains an iron-sulfur center, FAD and reduces NAD+, family 2 enzymes are membrane-bound, contain FMN and are oxidized by ubiquinone. Mechanism consists of 3 reaction phases. Different binding-mechanisms for enzyme the reaction-steps are suggested | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + acceptor | uses ubiquinone as electron acceptor | Escherichia coli | orotate + reduced acceptor | - |
? | |
(S)-dihydroorotate + acceptor | fourth step in synthesis of pyrimidine nucleotides | Escherichia coli | orotate + reduced acceptor | - |
? | |
(S)-dihydroorotate + ubiquinone | reaction studied with menadione, O2 and ferricyanide | Escherichia coli | orotate + ubiquinol | - |
? | |
dihydroorotate + acceptor | reaction intermediates, mechanism, pH-dependence of reaction | Escherichia coli | orotate + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
DHOD | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.7 | - |
S-dihydroorotate | pH 8.5, 4°C, with menadione | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 12.7 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Escherichia coli |